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KMID : 1134819970260050978
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Journal of the Korean Society of Food Science and Nutrition
1997 Volume.26 No. 5 p.978 ~ p.982
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Effects of Ganoderma lucidum on the IL - 1, TNF and IL - 12 Gene Expression of Macrophages
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Bae Ji-Hyun
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Abstract
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In order to investigate the immunomodulatory mechanism of Ganoderma lucidum, the effects of protein-bound polysaccharide of Ganoderma lucidum on the proliferation and cytokine gene expression of mouse peritoneal macrophages was studied. In the macrophage proliferation assay using the BrdU labeling reagent, the GLA component extracted from Ganoderma lucidum or GLB from the bud of Ganoderma lucidum were added to the medium at the concentration of 0 to 256㎍/ml. DNA synthesis of the macrophage was increased at 16㎍/ml of GLA and 64㎍/ml of GLB, respectively. In the reverse transcription polymerase chain reaction(RT-PCR), the cytokine(TNF, IL-1, and IL-12) gene and β-actin expression were also analyzed. 20㎍/ml of either GLA or GLB increased TNF and IL-1 expression of the macrophages.
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KEYWORD
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Ganoderma lucidum, macrophages, cytokine, gene expression
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